Journal: International journal of molecular medicine

Article Title: Long non‑coding RNA urothelial cancer associated 1 regulates radioresistance via the hexokinase 2/glycolytic pathway in cervical cancer.

doi: 10.3892/ijmm.2018.3778

Figure Lengend Snippet: Figure 4. SiHa‑IRR and HeLa‑IRR cells exhibit increased glycolysis. (A and B) Glucose consumption and lactate production analyses of IRR and parental cells. (C) Western blot analysis of the protein expression levels of HK2, HIF‑1α, GLUT‑1, GLUT‑4 and PKM in the IRR and parental cells. Data are presented as the means ± standard deviation from three separate experiments. *P<0.05, **P<0.01, ***P<0.001. GLUT, glucose transporter; HIF‑1α, hypoxia‑inducible factor 1α; HK2, hexokinase 2; IRR, irradiation‑resistant; PKM, pyruvate kinase muscle isozyme M2.

Article Snippet: The membranes were then incubated in blocking solution containing Tris-buffered saline containing 0.1% Tween (TBST; pH 7.4) and 5% (mass‐volume concentration) low‐fat milk at room temperature for 1 h. After being washed three times in TBST, the membranes were incubated with rabbit polyclonal immunoglobulin G anti‐human antibodies: HK2 (1:1,000, cat. no. 22029‐1‐AP), hypoxia‐inducible factor 1α (HIF-1α; 1:400, cat. no. 20960‐1‐AP), glucose transporter (GLUT)-1 (1:1,000, cat. no. 21829-1-AP), GLUT-4 (1:400, cat. no. 21048-1-AP), pyruvate kinase muscle isozyme M2 (PKM2; 1:1,000, cat. no. 15822‐1‐AP) and GAPDH (1:4,000, cat. no. 10494-1-AP) (ProteinTech Group, Inc., chicago, IL, USA) overnight at 4 ̊C.

Techniques: Western Blot, Expressing, Standard Deviation

Journal: International journal of molecular medicine

Article Title: Long non‑coding RNA urothelial cancer associated 1 regulates radioresistance via the hexokinase 2/glycolytic pathway in cervical cancer.

doi: 10.3892/ijmm.2018.3778

Figure Lengend Snippet: Figure 5. Inhibition of glycolysis by 2‑DG restores the radiosensitivity of SiHa‑IRR and HeLa‑IRR cells. (A and B) Glucose consumption and lactate produc- tion analyses of IRR cells treated with 2‑DG. (C) Western blot analysis of the protein expression levels of HK2, HIF‑1α, GLUT‑1, GLUT‑4 and PKM in the IRR cells treated with 2‑DG. (D and E) 2‑DG‑treated IRR cell survival fraction data were fitted to the single‑hit multitarget model. Data are presented as the means ± standard deviation from three separate experiments. **P<0.01, ***P<0.001. 2‑DG, 2‑deoxy‑D‑glucose; GLUT, glucose transporter; HIF‑1α, hypoxia‑inducible factor 1α; HK2, hexokinase 2; IRR, irradiation‑resistant; PKM, pyruvate kinase muscle isozyme M2.

Article Snippet: The membranes were then incubated in blocking solution containing Tris-buffered saline containing 0.1% Tween (TBST; pH 7.4) and 5% (mass‐volume concentration) low‐fat milk at room temperature for 1 h. After being washed three times in TBST, the membranes were incubated with rabbit polyclonal immunoglobulin G anti‐human antibodies: HK2 (1:1,000, cat. no. 22029‐1‐AP), hypoxia‐inducible factor 1α (HIF-1α; 1:400, cat. no. 20960‐1‐AP), glucose transporter (GLUT)-1 (1:1,000, cat. no. 21829-1-AP), GLUT-4 (1:400, cat. no. 21048-1-AP), pyruvate kinase muscle isozyme M2 (PKM2; 1:1,000, cat. no. 15822‐1‐AP) and GAPDH (1:4,000, cat. no. 10494-1-AP) (ProteinTech Group, Inc., chicago, IL, USA) overnight at 4 ̊C.

Techniques: Inhibition, Western Blot, Expressing, Standard Deviation

Journal: International journal of molecular medicine

Article Title: Long non‑coding RNA urothelial cancer associated 1 regulates radioresistance via the hexokinase 2/glycolytic pathway in cervical cancer.

doi: 10.3892/ijmm.2018.3778

Figure Lengend Snippet: Figure 7. UCA1 regulates the glycolytic pathway by targeting HK2 in cervical cancer cells. Western blot analysis of protein expression levels of HK2, HIF‑1α, GLUT‑1, GLUT‑4 and PKM in (A) SiHa‑IRR and HeLa‑IRR cells transfected with UCA1 siRNA, and (B) SiHa and HeLa cells transfected with pcDNA‑U plasmid. Data are presented as the means ± standard deviation from three separate experiments. *P<0.05, **P<0.01. GLUT, glucose transporter; HIF‑1α, hypoxia‑inducible factor 1α; HK2, hexokinase 2; IRR, irradiation‑resistant; NC, negative control; pcDNA‑M, pcDNA/Mock; pcDNA‑U, pcDNA/UCA1; PKM, pyruvate kinase muscle isozyme M2; siRNA, small interfering RNA; UCA1, urothelial cancer associated 1.

Article Snippet: The membranes were then incubated in blocking solution containing Tris-buffered saline containing 0.1% Tween (TBST; pH 7.4) and 5% (mass‐volume concentration) low‐fat milk at room temperature for 1 h. After being washed three times in TBST, the membranes were incubated with rabbit polyclonal immunoglobulin G anti‐human antibodies: HK2 (1:1,000, cat. no. 22029‐1‐AP), hypoxia‐inducible factor 1α (HIF-1α; 1:400, cat. no. 20960‐1‐AP), glucose transporter (GLUT)-1 (1:1,000, cat. no. 21829-1-AP), GLUT-4 (1:400, cat. no. 21048-1-AP), pyruvate kinase muscle isozyme M2 (PKM2; 1:1,000, cat. no. 15822‐1‐AP) and GAPDH (1:4,000, cat. no. 10494-1-AP) (ProteinTech Group, Inc., chicago, IL, USA) overnight at 4 ̊C.

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Standard Deviation, Negative Control, Small Interfering RNA